TABLE 1.
Primer sets for real-time PCRs
| Primer sequence | Primer position (nt)a | HPV 18 amplification region | Primer concn (μM) | Amplicon size (bp) |
|---|---|---|---|---|
| 5′-AGACACCGAAGGAAACCCTTT-3′ | 2821-2841 | E2 (amino terminus 1) | 0.25 | 223 |
| 5′-GCTTTATGTGCTTTACTTTTTGA-3′ | 3201-3043 | |||
| 5′-TGCAAGACACATGCGAGGAA-3′ | 3109-3129 | E2 (amino terminus 2) | 0.25 | 157 |
| 5′-CATGTTCCTGCATCAGTCATAT-3′ | 3244-3265 | |||
| 5′-AAAATATGGGAACACAGGTACG-3′ | 3359-3380 | E2 (hinge) | 0.25 | 192 |
| 5′-GCCGACGTCTGGCCGTAGGTCT-3′ | 3529-3550 | |||
| 5′-TACAGGCAACAACAAAAGACG-3′ | 3632-3652 | E2 (carboxyl terminus 1) | 0.25 | 177 |
| 5′-CCTGTTTTTTCATTGCCTGC-3′ | 3789-3808 | |||
| 5′-GTCACGAGCAATTAAGCGAC-3′ | 669-688 | E7 | 0.25 | 212 |
| 5′-CACAAAGGACAGGGTGTTCA-3′ | 861-880 | |||
| 5′-GCACGGCATCGTCACCAACT-3′ | 1283-1303 | Beta-actin | 0.125 | 142 |
| 5′-CATCTTCTCGCGGTTGGCCT-3′ | 1404-1424 |
a
nt, nucleotide. Position numbering is according to the HPV18 reference strain (GenBank accession no. NC_001357).