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(A) Expression of vtRNA1 in C127 cells infected with MHV-68. C127 cells were infected at an MOI of 5 PFU/cell, and at various times, the RNA was harvested and subjected to RT-PCR for vtRNA1. Experiments were carried out in the presence (+RT) or absence (−RT) of reverse transcriptase. (B) RNA was isolated from the virus stock and from infected cells at 0 hpi and subjected to RT-PCR for vtRNA1.
