FIG. 3.

VACV infection of CCR5^+/+ but not CCR5^−/− mice influences the T-cell responses in secondary lymphoid organs. Groups of female mice (n = 10), age 6 to 8 weeks, were infected with 10⁴ PFU of EGFP-VACV by intranasal inoculation. Mice were euthanized at the indicated times p.i., and CD3⁺ CD4⁺ and CD3⁺ CD8⁺ T-cell counts in the spleens (A and B) and mLNs (C and D) were determined by flow cytometry, using PE-Cy5-conjugated anti CD4/CD8 and APC-conjugated CD3 antibodies. ▪, total CCR5^+/+ CD4⁺/CD8⁺; ░⃞, total CCR5^−/− CD4⁺/CD8⁺. Data are shown as mean cell counts ± standard errors and are representative of two independent experiments. *, P < 0.05; **, P < 0.01.