FIG. 4.

VACV infection modulates CD11c⁺ DC and F4/80⁺ macrophage populations in the mLNs and spleens of CCR5^+/+ but not CCR5^−/− mice. CD11c⁺ and F4/80⁺ cell infiltration into the mLNs (A and C) and spleens (B and D) of EGFP-VACV-infected mice (10⁴ PFU) was determined by flow cytometry, using APC-conjugated anti-CD11c and PE-Cy5-conjugated anti-F4/80 antibodies. Data are presented as total cell number infiltrate of CD11c⁺/F4/80cells. ▪, total CCR5^+/+ CD11c⁺/F4/80⁺; ░⃞, total CCR5^−/− CD11c⁺/F4/80⁺. Data are shown as mean cell counts ± standard errors and are representative of two independent experiments. *, P < 0.05 **, P < 0.01.