FIG. 4.

The reovirus T1L M1 gene inhibits IFN induction of an ISRE promoter. HEK293 cells were transfected with an ISRE-luciferase reporter construct, a constitutively expressing Renilla luciferase construct, and the plasmids shown. At 24 hours posttransfection, cultures were either mock treated or treated with 1,000 U of IFN-β per ml for 6 h. Luciferase activity for each sample was normalized to the internal Renilla luciferase control, and the mean from duplicate samples was divided by that from the mock-treated samples to express the data as mean fold induction + standard deviation. A representative of three independent experiments is shown. Asterisks indicate significant differences from pCAGGs (Student's t test, P < 0.05). The M1-encoded μ2 protein was detected by immunoblotting using rabbit polyclonal anti-μ2.