FIG. 6.

The T1L M1 gene mediates nuclear accumulation of IRF9. L929 cell cultures were either mock treated or infected with the indicated reovirus strains at an MOI of 25 PFU per cell for 20 h and treated with 1,000 U per ml of IFN-α/β for 5 h. Cytoplasmic and nuclear protein extracts were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to a nylon membrane, and immunoblotted using rabbit polyclonal anti-IRF9, -STAT2, or -actin. Gels were scanned and band intensity quantified as indicated. The band migrating above STAT2 in T1L-infected cultures in panel D was identified as a reovirus protein by mass spectrometry (data not shown).