TABLE 1.
Repression of IFN-induced IRF7 mRNA segregates with the T1L M1 and S2 gene segments and is associated with induction of myocarditis
| Virusb | Reovirus gene segments and proteinsa
|
Myocarditise | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Outer capsid
|
Core
|
Nonstructural
|
|||||||||
| S1 | S4 | M2 | S2c | M1d | L1 | L2 | L3 | S3 | M3 | ||
| E3 | 3 | 3 | 1 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | − |
| EW26 | 1 | 1 | 3 | 3 | 1 | 3 | 3 | 1 | 1 | 1 | − |
| EW50 | 1 | 3 | 3 | 3 | 3 | 1 | 1 | 1 | 3 | 3 | − |
| DB93A | 1 | 1 | 3 | 3 | 3 | 1 | 3 | 3 | 1 | 3 | − |
| EW29 | 3 | 1 | 1 | 3 | 3 | 3 | 3 | 1 | 1 | 1 | − |
| T3D | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | − |
| DB95 | 3 | 3 | 3 | 3 | 3 | 1 | 3 | 3 | 1 | 3 | − |
| EW43 | 3 | 1 | 1 | 1 | 3 | 3 | 3 | 1 | 3 | 1 | − |
| 3HA1 | 1 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | ND |
| DB181 | 1 | 3 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | + |
| EW89 | 3 | 1 | 3 | 3 | 1 | 1 | 3 | 1 | 1 | 3 | + |
| EW60 | 1 | 1 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | + |
| EW100 | 3 | 1 | 3 | 1 | 1 | 3 | 3 | 1 | 3 | 3 | + |
| T1L | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | +/− |
| 8B | 3 | 1 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | + |
| 1HA3 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ND |
1, derivation from T1L; 3, derivation from T3D. Results from Fig. 2 were used for a nonparametric Kruskal-Wallis analysis to identify reovirus genes associated with inhibition of IFN signaling. P values for method A and method B (Fig. 2) at each of the two IFN doses were >0.05 (not significant) for all genes other than S2 and M1.
Viruses are listed in the same order as in Fig. 2.
T1L S2 is associated with inhibition of IFN signaling. Method A, P = 0.003 (1,000 U IFN) and P = 0.012 (100 U IFN). Method B, P = 0.007 (1,000 U IFN) and P = 0.039 (100 U IFN).
T1L M1 is associated with inhibition of IFN signaling. Method A, P = 0.006 (1,000 U IFN) and P = 0.006 (100 U IFN). Method B, P = 0.025 (1,000 U IFN) and P > 0.05 (100 U IFN).
Viruses are designated as myocarditic (+) or nonmyocarditic (−) based on references 60 and 61. ND, not determined. Induction of myocarditis is associated with inhibition of IFN signaling. Method A, P = 0.004 (1,000 U IFN) and P = 0.004 (100 U IFN). Method B, P = 0.004 (1,000 U IFN) and P = 0.004 (100 U IFN).