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Residues 182 to 247 are ID when attached to the CTD. (A) 15N-edited HSQC spectra of NP248-365 (CTD) (left) and NP182-365 (right) show additional resonances clustered in the middle of the spectrum of NP182-365. Axis units are ppm. (B) Size exclusion chromatogram of NP182-365. The corresponding apparent molecular weight was calculated from the equation log(MW) = 6.5404 − 0.1802 EV, where MW is the molecular weight in thousands and EV is the elution volume in milliliters.
