TABLE 1.

Binding coefficients for U₂₀ ssRNA to various regions of the SARS-CoV N protein^a

Buffera and region (aa)	Apparent Kd (μM)b	Hill coefficientb
Buffer A
1-181	2.98 ± 0.19	1.6 ± 0.15
45-181	9.81 ± 0.82	1.1 ± 0.09
45-247	2.73 ± 0.05	3.2 ± 0.16
182-365	1.35 ± 0.06	10.6 ± 1.0
248-365	10.2 ± 0.89	0.9 ± 0.06
248-422	1.62 ± 0.05	2.9 ± 0.22
45-365	0.74 ± 0.04	2.3 ± 0.26
Buffer B
45-181	9.40 ± 1.1	0.53 ± 0.04
248-365	9.30 ± 0.89	0.65 ± 0.04
Sumo-1-FL	15.6 ± 1.1	1.5 ± 0.14

^aBuffer A consists of 10 mM NaP_i, 50 mM NaCl, and 1 mM EDTA (pH 6.0). Buffer B consists of 50 mM NaP_i, 150 mM NaCl, and 1 mM EDTA (pH 7.4).

^bValues are averages for three individual experiments ± standard deviations.