FIG. 1.

MG132 inhibits late protein expression. (A) HeLa cells were mock infected or infected with VV65 at an MOI of 5 in the presence or absence of 10 μM MG132. VV65-infected samples were harvested at 4, 8, 12, 16, and 24 h, and mock-infected samples were harvested at 4 h and Western blotted with anti-I5L, anti-B5R, anti-Mcl-1, and anti-Bak. α, anti. (B) HeLa cells were infected with VV65 in the presence and absence of 40 μg/ml AraC to determine late expression for I5L and B5R. (C) Metabolic labeling of VV65-infected cells. HeLa cells were mock infected or infected with VV65 at an MOI of 5 and treated with either 10 μM MG132 or 40 μg/ml AraC. Cells were pulse-labeled with [³⁵S]methionine/cysteine at 4.5 h postinfection, and samples were analyzed by SDS-PAGE followed by autoradiography. Late proteins are marked with dots in the VV65-infected sample. (D) BGMK cells were infected with VV65 in the presence and absence of MG132, and plaques were visualized by staining with X-Gal.