FIG. 9.

Inhibition of the ubiquitin proteasome system dramatically affects poxvirus production. (A) HeLa cells were infected with VV65 at an MOI of 1, and samples were harvested at 4, 8, 12, and 24 h postinfection. Samples were collected in triplicate, and titers were determined on BGMK cells. (B) HeLa cells were infected with cowpox virus Brighton Red (CPV) at an MOI of 1. (C) HeLa cells were infected with ectromelia virus strain Moscow (EVM) at an MOI 1. (D) HeLa cells were infected with VV65 at an MOI 5 for 10 or 24 h or the presence of 10 μM MG132 for 10 h. Additionally, MG132 was removed by washout at 10 h and infection was allowed to progress for a further 8 h.