FIG. 5.

Serum NAb titers and vector antitumor efficacy in naïve and immunized hamsters. (a) Neutralization assay. A neutralization assay was performed to determine circulating anti-Ad NAb in different groups at different time points (1 day before the first i.t. injection, 20 days after the first i.t. injection, and at the time of sacrifice). NAb titers were determined by the highest dilution of serum that resulted in at least 50% inhibition of CPE when incubated with 100 PFU of INGN 007. (b) INGN 007 antitumor efficacy. Established HaK tumors (mean volume, 500 μl) were injected for six consecutive days with 1 × 10¹⁰ PFU of INGN 007 or buffer. Tumors were measured using digital calipers, and the mean tumor volume for 10 hamsters in each group is shown.