FIG. 2.

Tetherin inhibits alpharetrovirus, betaretrovirus, and deltaretrovirus release. Shown is a Western blot analysis of 293T cells and corresponding VLPs after the coexpression of Gag-myc or Gag-Pol proteins in the presence or absence of Vpu and various amounts of tetherin. RSV Gag (A) was revealed with anti-myc antibodies, MPMV Gag (B) and HERV-K Gag (C) were revealed using anti-CA antisera, and HTLV-1 Gag (D) was revealed with an anti-HTLV-1 p19 antibody. Numbers below each lane indicate values obtained upon densitometric scanning of VLP blots.