FIG. 6.

Quantitative analysis of colocalization between tetherin and nascent VLPs. Images were acquired at the focal plane representing the cell-coverslip interface. For each viral protein-GFP fusion, 10 pixel-square regions encompassing puncta or filaments of viral protein-GFP fluorescence were selected for three to five viral protein-GFP-expressing cells. The mean fluorescence intensities associated with viral protein-GFP (x axis) and tetherin (y axis) in each region are plotted. a.u., arbitrary units.