FIG. 1.

Restriction of CA mutants A92E and G94D is strengthened by arresting HeLa cells with aphidicolin. HeLa cells (A and B) or TE671 cells (C and D) were infected with wild-type or mutant virus in the presence (white bars) and absence (black bars) of aphidicolin. Cells were also treated with 5 μM Cs or vehicle (dimethyl sulfoxide) control as shown. HeLa (B) and TE671 (D) cells were infected with MLV as a control with or without aphidicolin, as shown. (E) HeLa cells transduced with empty vector (vector) or vector encoding a short hairpin RNA targeting CypA (sh CypA) were also infected with wild-type or mutant HIV-1 A92E or G94D in the presence (white bars) and absence (black bars) of aphidicolin, as shown. Infected cells were counted by fluorescence-activated cell sorting 48 h after infection, and infectious titers were calculated. HIV-1 infectivity is expressed as infectious units per ng reverse transcriptase activity. MLV titers are expressed as infectious units per milliliter. Errors are standard deviations of titers determined at two doses. Results are representative of at least two experiments performed using independent preparations of virus. wt, wild type; RT, reverse transcriptase; DMSO, dimethyl sulfoxide.