FIG. 6.

Effect of the dl359 mutation on the localization of late viral and total cell mRNAs. H1299 cells were either mock infected or infected with wt dl309 or the mutant dl359, as indicated on the left. At 24 hpi fixed cells were hybridized with either a fluorescent oligonucleotide complementary to the spliced tripartite leader (frames a, e, and i in A) or a fluorescent poly(dT) oligo (frames a, e, and i in B). Following hybridization, the late viral proteins were immunostained with an antibody recognizing the capsid proteins (frames b, f, and j in both panels). DAPI was used to stain the nucleus, as indicated on the figure. Images in the first three columns are combined at right (merge).