FIG. 3.

ISG15 UbE1L-interacting mutant R151A is attenuated in its ability to decrease Sindbis virus replication. Viral titers of dsTE12Q, mISG15 LRLRGG, or mISG15 R151A in brains, spleen, liver, lung, and serum of IFN-αβR^−/− mice infected for 3, 5, or 7 days. Data are pooled from two independent experiments with six mice per group. Error bars represent standard errors of the means, and the dashed line indicates the plaque assay limit of detection. Brackets denote statistically significant (P < 0.05) differences between indicated viruses.