FIG. 1.

Strategy for generating pseudotyped single-cycle SIVs expressing IFN-γ and a mock control. Deletions in full-length SIVmac239 proviral DNA are shown as shaded regions, and the inserted rat IFN-γ (Rγ) and GFP genes are shown as open boxes. An IFN-γ gene was also inserted in the antisense orientation and is designated aRγ. Pseudotyped SIVs or the mock control was generated by the transient cotransfection of 293T cells with pVSV-G and a plasmid encoding the defective proviral DNA or pLGRN. LTR, long terminal repeat; CMV, cytomegalovirus; pA, polyadenylation signal.