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Prominent Th1 polarization in dSIVLRγ-immunized animals was detected. The production of IFN-γ, IL-2, and IL-4 after SIVp55 Gag-specific stimulation or VSV-N nonspecific stimulation of splenocytes from immunized animals was demonstrated. Cytokine concentrations in supernatants of stimulated cells were measured by ELISA. Positive controls were ConA-stimulated splenocytes (results not shown). Bars represent the mean concentration for each group, and error bars represent SEM.
