TABLE 2.

Gag produced by single-cycle SIVs in different cell lines^a

Viral particle designation	Amt of Gag (ng/ml) produced in:
	HeLa cells	Rat-2 cells
Mock	<0.049b	<0.049b
dSIVR	0.67 ± 0.026	0.45 ± 0.089
dSIVLRγ	0.61 ± 0.0079	0.35 ± 0.063
dSIVHRγ	0.71 ± 0.043	0.35 ± 0.030

^aHeLa or Rat-2 cells were transduced with different constructs at an MOI of 0.5. Two days posttransduction, supernatants were collected and p27 Gag concentrations were measured by ELISA. The data shown are the mean values ± SEM for triplicate samples.

^bThe detection range for this assay is 0.049 to ∼0.784 ng/ml. The reading was under the detection limit.