FIG. 5.
Addition of LMB does not block the nuclear export of FMDV Lpro. LF-BK cells were infected at an MOI of 10 with A12-WT (panels 1, 3, 5, and 7) and the double SAP mutant A12#49 (panels 2, 4, 6, and 8). Where indicated, 200 nM LMB was added to the culture medium. At 6 hpi, cells were fixed and stained. For panels 1 to 4, viral protein Lpro was detected using a rabbit polyclonal Ab and an Alexa Fluor 488-conjugated secondary Ab. Viral protein VP1 was detected using mouse MAb 6HC4 and an Alexa Fluor 594-conjugated secondary Ab. For panels 5 to 8, p65/RelA was detected using rabbit polyclonal Ab Abcam RB-1638 and an Alexa Fluor 488-conjugated secondary Ab.