FIG. 8.

FMDV L^pro catalytic activity is required for p65/RelA degradation. IBRS-2 cells were infected with TMEV-Lb-containing WT or mutant C23A L^pro at an MOI of 10. At 24 hpi, p65/RelA and viral proteins were visualized by IFA. p65/RelA was detected with rabbit polyclonal Ab (RB-1638) and Alexa Fluor 488-conjugated secondary Ab. L^pro was visualized a rabbit polyclonal Ab and Alexa Fluor 488-conjugated secondary Ab. TMEV VP1 was detected with MAb DAmAb2 (28) and Alexa Fluor 594-conjugated secondary Ab.