TABLE 1.
Oligonucleotide primer and probe sequences for real-time RT-PCR
| Gene | Primer and probe setsa | Sequence | NCBI GenBank accession no. |
|---|---|---|---|
| GAPDH | Porcine GAPDH-327F | CGTCCCTGAGACACGATGGT | AF017079 |
| Porcine GAPDH-380R | CCCGATGCGGCCAAAT | ||
| Porcine GAPDH-348T | AAGGTCGGAGTGAACG | ||
| IFN-β | Porcine IFN-β-11F | AGTGCATCCTCCAAATCGCT | M86762 |
| Porcine IFN-β-69R | GCTCATGGAAAGAGCTGTGGT | ||
| Porcine IFN-β-32T | TCCTGATGTGTTTCTC | ||
| IRF7 | Porcine IRF7-418F | CTGCGATGGCTGGATGAA | TC224060 |
| Porcine IRF7-511R | TAAAGATGCGCGAGTCGGA | ||
| Porcine IRF7-450T | CCGCGTGCCCTGGAAGCACTT | ||
| Mx1 | Porcine Mx1-803F | GAGGTGGACCCCGAAGGA | M65087 |
| Porcine Mx1-859R | CACCAGATCCGGCTTCGT | ||
| Porcine Mx1-824T | AGGACCATCGGGATC | ||
| RANTES | Porcine RANTES-54F | TGGCAGCAGTCGTCTTTATCA | F14636 |
| Porcine RANTES-125R | CCCGCACCCATTTCTTCTC | ||
| Porcine RANTES-101T | TGGCACACACCTGGCGGTTCTTTC | ||
| TNF-α | Porcine TNF-α-338F | TGGCCCCTTGAGCATCA | NM214022 |
| Porcine TNF-α-405R | CGGGCTTATCTGAGGTTTGAGA | ||
| Porcine TNF-α-356T | CCCTCTGGCCCAAGGACTCAGATCA |
a
As reported by Moraes et al. (37). Numbering in the primer name refers to the nucleotide positions in the specific gene coding region. Suffix letters: F, forward primer; R, reverse primer; T, TaqMan FAM-MGB probe.