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Anti-HA MAbs recognize tagged monomeric gp120. Solubilized monomeric gp120 from viral lysates was captured onto ELISA plate wells using an anti-gp120 sheep antibody to the C5 region and probed with MAbs 3F10 and 16B12, starting at a concentration of 25 μg/ml. (A) JRCSF gp120. (B) HXB2 gp120. All values are the means from replicate wells.
