FIG. 2.

RV encoding bicistronic RL-IRES-FL reporter genes. (A) Growth of IRES reporter RVs in BSR T7/5 cells. Cells were infected with the indicated viruses at an MOI of 0.1, and infectious supernatant virus titers were determined at the indicated time points. As a control a virus containing an additional monocistronic gene downstream of G (SAD G GFP) was used for comparison. (B) Cell lines from nonprimate (upper panel) and primate species (lower panel), including cells of neuronal origin (underlined) were infected with the recombinant SAD RL-IRES-FL reporter viruses containing the IRES of PV Mahoney (light gray), HRV type 2 (black), or FMDV (dark gray). At 48 h p.i. RL and FL activities were measured using the dual luciferase reporter system (Promega). The ratio of activity of FL to RL with SAD RL-PV-FL was set as 100%. Data for every cell line are from at least two independent experiments, each of which included three parallel infections. Error bars indicate standard deviations. P ≤ 0.001 for all cells and viruses.