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NKT cells in pigtail macaques. (A) Gating strategy to define NKT cells in peripheral blood and proportions of CD4+ and CD8+ subsets. SSC, side scatter; FSC, forward scatter. (B) An empty CD1d tetramer (without α-GalCer) is shown to validate the staining of the α-GalCer/CD1d tetramer. (C) Expression of IFN-γ following in vitro stimulation is shown for two animals. PMA, phorbol myristate acetate.
