TABLE 1.
Sequences of primers used in this study to generate SeV mutant L genes
| L mutant or primer | L change | Silent site | Primer sequencea |
|---|---|---|---|
| L-1782A | K1782A | AfeI | CATCAACAGTACTAGCTGCTTGgcAGCGCTTGAACTTACCTACCTATT (+) |
| CAATAGGTAGGTAAGTTCAAGCGCTGCCAAGCAGCTAGTACTGTTGAT (−) | |||
| L-1804A | G1804A | EaeI | GATAGGCTATATTTGGccGAAGGAGCTGGGGCCATG (+) |
| CATGGCCCCAGCTCCTTCGGCCAAATATAGCCTATC (−) | |||
| L-1805A | E1805A | AvrII | GATAAAGATAGGCTATACCTAGGGGcAGGAGCTGGGGCCATG (+) |
| GCATGGCCCCAGCTCCTGCCCCTAGGTATAGCCTATCTTTAT (−) | |||
| L-1805V | E1805V | AvrII | GATAAAGATAGGCTATACCTAGGGGtAGGAGCTGGGGCCATG (+) |
| GCATGGCCCCAGCTCCTACCCCTAGGTATAGCCTATCTTTAT (−) | |||
| L-1806A | G1806A | AvrII | GATAAAGATAGGCTATACCTAGGGGAAGcAGCTGGGGCCATGCTTTC (+) |
| GAAAGCATGGCCCCAGCTTCCCCTAGGTATAGCCTATCTTTATC (−) | |||
| L-1804A/1806A | G1804A/G1806A | PstI | GATAGGCTATATTTAGcGGAAGctGCAGGGGCCATGCTTTC (+) |
| GAAAGCATGGCCCCTGCAGCTTCCGCTAAATATAGCCTATC (−) | |||
| L-ΔVI | Deletion of aa 1777-1976 | None | GGCTCTTTGGCATCAACCTTCTATCGAGGCACCCC (+) |
| GGGGTGCCTCGATAGAAGGTTGATGCCAAAGAGCC (−) | |||
| Upstream primer VG19 for cloning and sequencing | CATACCTATGCAGCTTGGCAGAGA (+) | ||
| Downstream primer VG20 for cloning and sequencing | TAACCCTCAGGTTCCTGATCTCAC (−) |
a
Lowercase letters show substituted nucleotides resulting in the amino acid change. The underlined nucleotides represent alanine codons generated as a result of site-directed mutagenesis. +, plus sense; −, antisense.