FIG. 2.

Detection of free serum WHsAg during the course of acute, self-limited WHV infection in adult woodchucks. (A) Standard dilutions of purified WHsAg and reference serum from chronic WHV carrier woodchuck F5413. Free WHsAg circulating in serum was quantitated by Laurell electrophoresis (electroimmunodiffusion). Using endpoint titration, it was estimated that the reference serum contained 729 μg WHsAg per ml. (B) Dilutions (1:5) of serum from woodchuck F3538 obtained at different time points during the course of WHV infection. Values were compared against values for a 1:10 dilution of the reference serum from woodchuck F5413. The cutoff of the assay was defined as 2 μg/ml.