FIG. 3.

Detection of circulating immune complexes during the course of acute, self-limited WHV infection in adult woodchucks. (A) Woodchuck F3538. (B) Woodchuck F3539. (C) Woodchuck 3524. (D) Woodchuck 3391. CICs in serum were detected by PEG precipitation followed by Western blotting with rabbit anti-WHs antiserum. Purified serum WHsAg (0.5 μg) was used as a standard. IgG, light (25 kDa) and heavy (50 kDa) chains of IgG; MWHs, WHV middle (pre-S2) surface protein; SWHs, WHV small/major (S) surface protein. Arrows in D indicate CICs detected in the serum of woodchuck F3391 at 4 weeks postinfection.