FIG. 6.

Comparison of the S, M, and L surface proteins of WHsAg within subviral particles isolated from sera of adult woodchucks with acute, self-limited WHV infection and woodchucks with established chronic WHV infection. Subviral particles isolated from serum were separated by SDS-PAGE, and the S, M, and L proteins of WHsAg were detected by silver staining using 0.5 μg of subviral particles (A) or by Western blotting with a polyclonal rabbit anti-WHs antiserum using 1.0 μg of subviral particles (B). LWHs, WHV large (pre-S1) surface protein; MWHs, WHV middle (pre-S2) surface protein; SWHs, WHV small/major (S) surface protein.