FIG. 2.

ALI cultures of BAEC. (A) Pseudostratified epithelium of BAEC grown for 2 weeks under ALI conditions and visualized by DAPI staining and confocal laser scanning microscopy. (B) Mucociliary differentiation of 2-week-old ALI cultures of BAEC. Cilia were stained by an antibody recognizing β-tubulin (red). Mucus-producing cells were detected by an antibody specific for mucin-5AC. (C) Distribution of ciliated cells within the respiratory epithelium. At 2 weeks after ALI culturing, BAEC were stained for cytoceratin (green), an epithelial cell marker, and for β-tubulin to detect cilia (red).