FIG. 4.
Vitality of PCLS 1 day after preparation. (A) Bronchoconstriction. PCLS prepared from the bronchioli and cultured for 1 day were treated with different concentrations of methacholine. The addition of methacholine at a concentration of 10−6 mol/liter induced bronchoconstriction within seconds (lower left panel). The removal of methacholine resulted in a reversion of this effect (lower right panel). (B) Live/dead viability assay. Live cells are shown in green; dead cells are shown in red. The cells of the respiratory epithelium facing the airway are indicated by a white arrow (top panel). The bottom panel shows alveoli.