FIG. 6.

Reductions in Mfrns affect the expression of a 5′ IRE-GFP reporter construct. (A) MEL cells were differentiated or not as described in the legend to Fig. 4B. On day 2 of differentiation, MEL cells were treated with oligonucleotides specific to Mfrn1 (si-Mfrn1) or Mfrn2 (si-Mfrn2). Forty-eight hours later (day 4 of differentiation), the cells were transfected with a 5′ IRE-GFP construct. The cells were harvested 24 h later, and the GFP fluorescence intensity was determined by flow cytometry. The data are expressed as arbitrary units of fluorescence. Error bars represent the standard deviations of the results from two independent experiments. (B) Cells treated as described for panel A were lysed, and Western blot analysis was performed using anti-GFP and antitubulin antibodies.