FIG. 6.
Elg1 and Rad1 are essential for the ILT pathway, while Dnl4, Rad24, Ctf18, Nej1, Yku70, and recombination-proficient RPA are dispensable. For all triple mutants of the indicated relevant genotype, the telomere structure is shown from the onset of senescence, triggered after sporulation of the diploids (which were already recombining in type II-ALT), to death (as indicated) or during the indicated number of generations. The arrowheads indicate the size of the bulk of wild-type telomeres. Three experiments were performed for the nej1Δ and dnl4Δ mutants, four for the rad24Δ and ctf18Δ mutants, five for the rfa1-t11 mutant, and six for the elg1Δ, rad1Δ, and yku70Δ mutants. A TG1-3 32P-labeled probe was used with XhoI cutting.