FIG. 9.

Stat3 involvement in breast cancer proliferation in vitro. (A) C4HD cells were transiently transfected with the Stat3Y705-F vector, with the empty pRc/CMV vector, or with siRNAs before HRG treatment for 48 h. Incorporation of [³H]thymidine was used as a measure of DNA synthesis. Data are presented as means ± standard deviations (P < 0.001 for b versus a and c versus b). The experiment shown is representative of a total of three. (B) C4HD and T47D cells were transfected with the indicated siRNAs before HRG treatment for 48 h and were then stained with PI and analyzed for cell cycle distribution by flow cytometry. The percentages of total cells in the cell cycle phases are indicated. Mock, cells incubated with the transfection reagent. The experiments shown are representative of a total of three.