FIG. 7.

Effects of inhibition of microtubule assembly on Gli2 transcriptional activity. (A) Dose response of TN16. 2T3 cells transfected with a Gli reporter gene, 8×3′Gli-BSδ5/LucII, were treated with TN16 at the indicated doses for 24 h. Reporter luciferase activity was quantitated and normalized to activity of cotransfected β-Gal. (B and C) Potentiation of TN16 effects by Gli2. 2T3 cells were cotransfected with a Gli2 expression vector and the 8×3′Gli-BSδ5/LucII reporter (B) or −2712/+165-Luc reporter (C) and then treated with TN16 (1.0 μM) for 24 h. Luciferase activity of the Gli reporter (B) or BMP-2 promoter reporter (C) were measured with β-Gal normalization. *, P < 0.01 versus control; #, P < 0.05 versus Gli2.