FIG. 2.

Reexpression of PTEN or inhibition of PI3K with LY294002 induces type II cells to undergo type I-like kinetics of caspase-3 activation. Jurkat cells were pretreated for 2 h with DMSO as a control (A) or with 10 μM LY294002 (B). Cells were stimulated with 250 ng/ml anti-Fas (CH11) antibody in 0.1% serum for the indicated times. Immunoblot analysis detecting cleaved PARP (a marker of caspase-3 activation), phospho-PKB (pSer473), and total PKB levels is shown. (C) Time course of anti-Fas-induced apoptosis of Jurkat-T cells transiently transfected with vector alone or wild-type PTEN. The data represent the mean and SEM percentages of the maximum number of positive cells expressing intracellular active caspase-3. The results shown are representative of anti-Fas stimulations performed in triplicate. ***, P < 0.0005.