FIG. 1.

Akt translocates to the nucleus during apoptin-induced cell death. (A) MCF-7 cells were transfected with GFP-tagged apoptin or a vector control. At 18 h posttransfection, the localization of Akt in either the absence or presence of apoptin in MCF-7 cells was detected by confocal microscopy after immunostaining with anti-Akt and Cy3-conjugated secondary antibody. DAPI (4′,6-diamidino-2-phenylindole) was used to counterstain nuclei, and the images were overlaid to determine the Akt localization within the cell. (B) MCF-7 cells were transfected with different apoptin mutants, and the localization of Akt and apoptin was detected by immunocytochemistry.