FIG. 1.

SIRT1 is downregulated by HFD in adipose tissue. (A and B) Reduced expression of SIRT1 in mice on HFD. The epididymal fat (Adipo) or SVF collected from NC- or HFD-fed mice, and then immunoblotting (IB) (A) or quantitative real-time-PCR (B) was performed as described in Materials and Methods. (A) The graph shows the means ± the standard error(s) of the mean (SEM) from three independent experiments, and the values are expressed as percentage of control in SIRT1 expression compared to those observed in adipose tissue form NC-fed mice. (B) After the mRNA expression differences were normalized to a standard housekeeping gene (GAPDH) mRNA level; data are presented as the relative expression. *, P < 0.05 (NC versus HFD).