FIG. 3.

SIRT1 is required for repression of proinflammatory gene expression. Knockdown of SIRT1 increased inflammatory gene expression was examined. At 24 h after electroporation with control (ctrl) or SIRT1 (SIRT) siRNA, cells were stimulated with 10 ng of TNF-α/ml for 24 h. Total RNA was purified, and then quantitative real-time-PCR was performed as described in Materials and Methods. After the mRNA expression differences were normalized to the GAPDH mRNA level, data are presented as the relative expression. Error bars represent the means ± the SEM from four independent experiments. *, P < 0.05 (control versus SIRT1 siRNA). ND, not detected.