N nucleotides in DJH joints derived from E10 to E12 BALB/c embryos. On the left, the frequencies of N+ DJH joints in adults (black), newborns (gray), WT embryos (hatched), and RAG2+/− embryos derived from BALB/c male × BALB/c.RAG2−/− female matings (crosshatched) are shown. The distribution of ORFs (circled numbers) among N+ DJH joints of adults and embryo DJH joints is displayed on the right. *, P < 0.05; **, P < 0.01; ***, P < 0.001, ns, not significant.