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. 2008 Dec 22;29(5):1202–1211. doi: 10.1128/MCB.01496-08

FIG. 4.

FIG. 4.

TbMTAP is required for SL processing complex formation. (A) Western blot probing for TbMTr1-HA fusion protein using polyclonal rabbit HA antibody in HA.11 monoclonal antibody immunoprecipitation input (I), supernatant (S), and pellet (P) from TbMTr1−/HA and TbMTr1−/HA TbMTAP−/− cells. The asterisk indicates the nonspecific band detected by the polyclonal antibody. (B) Primer extension using oligonucleotide complementary to the SLA1 snoRNA from 2% input (I), 2% supernatant (S), and 10% pellet (P) RNA isolated by TbMTr1-HA immunoprecipitation from TbMTr1−/HA and TbMTr1−/HA TbMTAP−/− cells. (C) Western blot with monoclonal anti-HA antibody probing alternate fractions of a 2-to-20% sucrose gradient loaded with nuclear extracts from either TbMTr1−/HA (top) or TbMTr1−/HA TbMTAP−/− cells (bottom). Diamonds indicate the following protein standards: soybean trypsin inhibitor (20.1 kDa), bovine serum albumin (67 kDa), lactate dehydrogenase (140 kDa), and catalase (232 kDa).