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. 2009 Feb 26;4(2):e4588. doi: 10.1371/journal.pone.0004588

Figure 1. IFNγ-induced inhibition of C. trachomatis growth in WT MEFs.

Figure 1

Host cells were infected for 48 h with C. trachomatis or C. muridarum (MOI 1) and simultaneously treated with 100 U or 200 U/ml IFNγ or left untreated (control). (A) and (C) Immunofluorescence micrographs of MEFs infected with C. trachomatis or C. muridarum, respectively, stained with Chlamydia-IMAGEN kit. Chlamydiae (green), Host cells (red). Cytokine treatment resulted in a low number of detectable small inclusions in C. trachomatis infected cells only. Images taken using the same magnification. (B) and (D) Influence of IFNγ on development of infectious progeny. The yield of C. trachomatis (B), but not C. muridarum (D), infectious progeny decreased considerably upon IFNγ stimulation, Infectivity percentage calculated as follows: IFU/ml estimated for each treated monolayer / IFU/ml of control cells ×100. Infectivity expressed as a percentage of control cells ±standard deviation (SD) from three independent experiments (n = 3). WT, wild type; Ctr, C. trachomatis; Cmur, C. muridarum.