Fig. 5.

Expression of musashi-1 (A) and doublecortin and CaM kinase-like-1 (DCAMKL-1) (B) by colonic crypt epithelial cells adherent to colonic myofibroblasts. Isolated and disaggregated colonic crypt epithelial cells were applied to monolayers of primary colonic myofibroblasts and cultured for 30 min, followed by removal of nonadherent cells by washing. Immunocytochemical studies were undertaken on the cocultures using anti-musashi-1 (A) or anti-DCAMKL-1 (B) antibody. In A, musashi-1-expressing cells are stained brown and are seen close to the larger hematoxylin-stained nuclei of myofibroblasts (*).