Fig. 6.

Integrin-β₁ expression by isolated and disaggregated colonic crypt epithelial cells (A) and effect of blocking integrin-β₁ on the adherence of isolated colonic crypt epithelial cells to myofibroblasts (B). A: epithelial cells were labeled with phycoerythrin (PE)-conjugated anti-β₁-integrin antibody (line without filling) or appropriate isotype control antibody (line with filling). The figure is representative of 5 experiments using cells isolated from colonic mucosal samples of different subjects. B: isolated and disaggregated colonic crypt epithelial cells were incubated (for 30 min) with anti-β₁-integrin antibody or control antibody before coculture with monolayers of primary colonic myofibroblasts for 30 min. After washing, adherent cells were counted and are expressed per square centimeter. The figure represents data from 3 experiments.