Fig. 2.

Histones as potential substrates for MKP-1. Anti-MKP-1 antibody was used to immunoprecipitate (CS) mutant-substrate complex from PMA-treated COS-1 cell extract. Coomassie-stained SDS-PAGE analysis shows that the (CS) mutant, but not (WT) mutant, efficiently immuno-precipitated three specific bands labeled 1, 2, and 3.