Fig. 3.

Histone H3 is associated with MKP-1 in endothelial cells (EC). A: human EC were infected with myc-tagged, MKP-1 (WT) or (CS) expressing lentivirus and then stimulated with vascular endothelial growth factor (VEGF). The protein complex was precipitated with an agarose-conjugated anti-MKP-1 antibody and immunoblotted with an anti-histone H3 antibody. Myc-tagged MKP-1 protein was detected using a myc-specific antibody. B: in a reverse experiment, myc-tagged (CS) mutant, not (WT) was coprecipitated when histone H3 was immunoprecipitated using an anti-histone H3 antibody.