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Thrombin and VEGF stimulation decrease phosphorylation of histone H3 (Ser-10) in human EC. A: human EC treated with, VEGF (10 ng/ml), or thrombin receptor activating peptide (TRAP, 100 μM) over a 3-h time course. Immunoblot analysis was performed using a specific anti-histone H3 (phospho-Ser-10) antibody. Immunoblots of total histone H3 was used as loading controls. These blots represent 3 independent experiments.
