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. 2008 Aug;24(8):1087–1095. doi: 10.1089/aid.2008.0041

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Copyright 2008, Mary Ann Liebert, Inc.

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FIG. 3. — Subsets of proliferating bulk CD4⁺ and CD8⁺ T lymphocytes express Foxp3 after anti-CD3 stimulation, with enhancement by added interleukin (IL)-2. Peripheral blood mononuclear cells from eight healthy (HIV-1-uninfected) subjects were assessed for proliferation after stimulation with anti-CD3 antibody in the absence and presence of added IL-2. (A, B) Representative plots are shown for each of the four conditions (Uns = unstimulated; Uns + IL-2 = unstimulated with added 50 U/ml IL-2; St = stimulated by anti-CD3 antibody; St + IL-2 = stimulated with added 50 U/ml IL-2). Proliferation was determined by carboxyfluorescein succinyl ester dilution assessment by flow cytometry, with costaining of the CD4⁺ (A) and CD8⁺ (B) T lymphocytes for CD25 and Foxp3. (C) The results are summarized for all eight subjects. (D) Foxp3 were generally coexpressed in all analyses; a representative contour plot is shown to demonstrate coexpression.