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. 2008 Dec 5;296(2):H380–H388. doi: 10.1152/ajpheart.00225.2008

Fig. 8.

Fig. 8.

Mu-36 and leptin attenuate HG-induced p53 activity and expression of apoptosis-related factors. A: representative Western blot analysis showing p53 phospho-Ser392 and Bax expression in lysates prepared from ARVMs. Protein extracts from WT ARVMs and ARVMs transduced with GFP and mu-36 were separated by PAGE and nitrocellulose blots probed with antibodies to cleaved caspase-3. A, middle and bottom: densitometric analysis for phospho-Ser36 and Bax expression. Data represent 5 independent experiments for WT and mu-36 at 5 and 25 mM glucose: means ± SD and *P ≤ 0.05. B: representative Western blot analysis showing p53 phospho-Ser392 and Bax expression in lysates prepared from hearts of WT and Akita mice expressing rAAV-GFP (GFP) or rAAV-Lep (LEP). Protein extracts from WT, GFP, and LEP groups were separated by PAGE and nitrocellulose blots probed with antibodies to p53 phospho-Ser392 and Bax as in A. B, middle and bottom: densitometric analyses for p53 phospho-Ser392 and Bax expression. Data represent 4 to 5 independent experiments: means ± SD and *P ≤ 0.05.